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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Crosstalk between glial and glioblastoma cells triggers the “go-or-grow” phenotype of tumor cells

Fig. 1

Experimental design and glial cells phenotype in the presence of GBM cells secretome. a. Diagram of glial cells-priming experimental setup. GL261 cells were cultured for 24 h to generate tumor-conditioned media (shown in blue). Glial cells were incubated for 3 days in GL261-conditioned medium (i.e., exposed) or non-conditioned medium (i.e., unexposed). Fresh non-conditioned medium was then added to glial cells (unprimed and primed). After 24 h, conditioned medium from unprimed (light red) or primed (dark red) glial cells was added to GL261 murine GBM cells. Glial cells were analyzed for cell viability and GFAP marker and proteomics characterization of the secretome. Functional and mechanistic assays were performed in GL261 cells. b. MTT cell viability assay in glial cells unexposed or exposed to GL261 CM. c. Immunocytochemistry staining for GFAP (left) and quantification of GFAP-positive cells (right) in glial cells unexposed or exposed to GL261 CM. Abbreviations: GBM, glioblastoma; DMEM, Dulbecco’s Modified Eagle Medium; FBS, Fetal Bovine Serum; CM, conditioned media; IF, immunofluorescence; GFAP, Glial Fibrillary Acidic Protein. Results are representative of at least three independent experiments, performed in triplicates (data points represent mean + SEM)

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