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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: ARF1 recruits RAC1 to leading edge in neutrophil chemotaxis

Fig. 1

Independence of GBF1 in the translocation of PAK1, αPIX, and Gβγ to the leading edges. (a) Expression pattern of proteins in cells treated with GBF1 siRNAs . Cells transfected with siRNA against GBF1 or an irrelevant RNA duplex (Irr) were analyzed for expression of the indicated proteins by immunoblotting of the lysates (10 μg each). Data are representative of three independent experiments. (b-g) Subcellular localization of αPIX, PAK1, and Gβ. Differentiated HL-60 cells, transfected with GBF1 siRNA or Irr, were incubated with or without fMLP for 15 min, and subjected to anti-αPIX (b), anti-PAK1 (d), or anti-Gβ immunostaining (f), and percentages of αPIX molecules (c), PAK1 molecules (e), or Gβ molecules (g) translocated to the leading edges in fMLP-stimulated cells were calculated. F-actin was visualized by Texas Red-phalloidin. Data are representative images of three independent experiments (b, d, and f), and >25 cells were analyzed in three independent experiments (c, e, and g). Error bars, SEM (c, e, and g). ** p < 0.01 and * p < 0.05 compared with the Irr control. Bars, 10 μm (b, d, and f)

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