Skip to main content
Fig. 3 | Cell Communication and Signaling

Fig. 3

From: CLCA1 suppresses colorectal cancer aggressiveness via inhibition of the Wnt/beta-catenin signaling pathway

Fig. 3

Increased expression level of CLCA1 suppresses CRC cell growth in vitro. a Western blotting analysis of CLCA1 in the SW620 cell line transfected with CLCA1 CRISPR/Cas9-activation and -knockout plasmids. b qRT-PCR analysis of CLCA1 in the SW620 cell line transfected with CLCA1 CRISPR/Cas9-activation plasmids. c qRT-PCR analysis of CLCA1 in SW620 cell line transfected with CLCA1 CRISPR/Cas9-knockout plasmids. d Growth curves of the CLCA1-ACT and CLCA1-ACT-NC group. e Growth curves of the CLCA1-KO and CLCA1-KO-NC group. Abbreviations: qRT-PCR, quantitative reverse transcription polymerase chain reaction; CRISPR/Cas9, the Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-associated protein system; CLCA1-ACT, CLCA1-upregulated cells; CLCA1-ACT-NC, negative control of CLCA1-upregulated cells; CLCA1-KO, CLCA1-knockout cells; CLCA1-KO-NC, negative control of CLCA1-knockout cells. *P < 0.05, t-test; ***P < 0.001, t-test

Back to article page

Cell Communication and Signaling

ISSN: 1478-811X