Fig. 2

CLCA1 serum concentration and CLCA1 mRNA expression level are inversely correlated with CRC metastasis. a ELISA results showed that the CLCA1 serum expression level was significantly lower in CRC patients than in healthy controls (1.48 ± 1.06 ng/mL vs 1.06 ± 0.73 ng/mL, P = 0.0018, t-test). b Compared to that in patients with early stage disease (TNM stage I and II), the CLCA1 serum expression level was markedly reduced in patients with local or distant metastasis (TNM stage III and IV) (1.24 ± 0.86 ng/mL vs 0.88 ± 0.53 ng/mL, P = 0.013, t-test). c CLCA1 mRNA level in CRC samples from TCGA database. Abbreviations: ELISA, enzyme-linked immunosorbent assay; TCGA, the Cancer Genome Atlas