Fig. 8

a-d. Altered expression of phagocytosis markers in CRAMP-deficient microglial cells after bacterial stimulation. Microglial cells from CRAMP-KO or wild-type (WT) mice were incubated with bacterial supernatants of Gram-positive S. pneumoniae (SP) or Gram-negative N. meningitidis (NM) and bacterial cell wall components lipopolysaccharide (LPS) or peptidoglycan (PGN) for 24 h. mRNA expression levels of receptor for advanced glycation endproducts (RAGE; a), macrophage receptor with collagenous structure (MARCO; b), phospholipase D1 (PLD1; c) and PLD2 (d) were determined by real-time RT-PCR. Data were assessed from five independent experiments in duplicate. Statistical significance is marked as * - p < 0.05, ** - p < 0.01 or *** - p < 0.0001 (two-way ANOVA test followed by Bonferroni’s multiple-comparison test)