Fig. 6

a-e. Reduced heme oxygenase-1 induction in CRAMP-KO glial cells after bacterial stimulation. Astrocytes (a, c) and microglial cells (b, d) from CRAMP-knockout (KO) or wild-type (WT) mice were incubated with bacterial supernatants of Gram-positive S. pneumoniae (SP) or Gram-negative N. meningitidis (NM) and bacterial cell wall components lipopolysaccharide (LPS) or peptidoglycan (PGN) for 6 or 24 h. Microglial cells (e) from CRAMP-KO or WT mice were incubated with 1, 2 or 10 μM mouse CRAMP with or without NM for 6 h. Fluorescence intensity of the whole cell was measured and calculated as the ratio of untreated control cells to treated cells. The experiment was independently performed at least three times. The immunofluorescence results were calculated for >20 separate cells per experiment. Statistical significance is marked as * - p < 0.05, ** - p < 0.01 or *** - p < 0.0001 (two-way ANOVA test followed by Bonferroni’s multiple-comparison test)