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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Osteoglycin promotes meningioma development through downregulation of NF2 and activation of mTOR signaling

Fig. 2

OGN overexpression promotes cell proliferation and tumor cell colony formation. a OGN mRNA is expressed in IOMM-Lee meningioma cells stably transfected with OGN vector as compared to cells transfected with control vector. b Western blot and densitometry analysis reveals significant expression of OGN protein in meningioma cells stably transfected with OGN vector. c Immunohistochemistry profile of OGN protein expression in control and OGN-expressing cells, scale bar 50 μm. d OGN-expressing cells demonstrate significantly increased proliferation at 72 h after plating, compared to control cells, as evaluated by WST assay. e, f After 18 days of incubation in soft agar, OGN-expressing cells demonstrate significantly increased colony formation, scale bar 100 μm. g Western blotting and densitometry analysis reveals higher expression of cell cycle markers, cyclin D1, cyclin A2, and cyclin B1 in OGN cells compared with control cells. *p < 0.05

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