Fig. 4

Waixenicin A blocks endogenous TRPM7-like currents in primary mouse colon epithelial cells. Primary mouse colon epithelial cells (MCECs) were established as described in the method section and investigated by whole-cell patch clamp technique for inhibition by waixA and 2-APB. a Average normalized time course of TRPM7 current development in MCECs in the presence of 390 μM [Mg²⁺]_i without (n = 5) and with 10 μM waixA external application as indicated by the black bar (n = 6). b I/V relationships from a representative cell obtained at 1000 s from data in (a). c Average normalized time course of TRPM7-like current development in MCECs in the presence of 0 μM [Mg²⁺]_i without (n = 6) and with 10 μM waixA application (black bar; n = 5). d I/V relationships from a representative cell obtained at 500 s from data in (c). e Average normalized time course of TRPM7-like currents. At the time indicated by the black bar, cells were superfused with external solution supplemented with 100 μM 2-APB (n = 4; [Mg²⁺] _i  = 0 μM). f I/V relationships from a representative cell displayed before (a) or after (b) 2-APB application and after 2-APB washout (c)