Fig. 2

Waixenicin A Mg²⁺-dependently blocks endogenous TRPM7-like currents in HT-29. a Averaged normalized time course of TRPM7-like current development in the presence of 0 μM [Mg²⁺]_i without (n = 11) and with 10 μM waixenicin A (waixA) application (n = 10) as indicated by the black bar. b I/V relationships from a representative cell obtained at 1000 s and taken from data in (a). c Averaged normalized time course of TRPM7-like current development in the presence of 780 μM [Mg²⁺]_i without (n = 10) and with 10 μM waixA application as indicated by the black bar (n = 10). d I/V relationships from representative cells obtained at 1000 s and taken from data in (c). e Dose-response curve for inhibition of TRPM7-like currents by increasing concentrations of externally applied waixA in 0 μM or 780 μM [Mg²⁺]_i. Currents were extracted at +80 mV at 900 s, normalized to current at 500 s, plotted against waixA concentrations, and approximated by a dose-response curve fit. f Dose-response curve for [Mg²⁺]_i with externally applied 10 μM waixA at 500 s and for 500 s. Currents were extracted at +80 mV at 900 s, normalized to current at 500 s, plotted against [Mg²⁺]_i, and approximated by a dose-response curve fit (n = 4–10)