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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Inhibition of TRPM7 suppresses cell proliferation of colon adenocarcinoma in vitro and induces hypomagnesemia in vivo without affecting azoxymethane-induced early colon cancer in mice

Fig. 1

Endogenous TRPM7-like currents in HT-29. a RT-PCR analysis of TRPM6 and TRPM7 mRNA expression. GAPDH was used as the housekeeping gene. b Percentage expression of TRPM6 or TRPM7 mRNA to the housekeeping gene β-actin was represented by q-PCR analysis (n = 3). c Average normalized time course of TRPM7-like current development in HT-29 at various [Mg2+]i (n = 6–12). Decrease of [Mg2+]i from 1600 to 0 μM allowed TRPM7-like current development in a dose-dependent manner. d Dose-response curve for [Mg2+]i from data in C. Currents were extracted at +80 mV at 800 s, normalized to cell size in pA/pF, averaged and plotted against [Mg2+]i, and approximated by dose-response curve fit (IC50 = 600 μM; Hill coefficient = 1). e Current-voltage (I/V) relationships f TRPM7-like currents from representative HT-29 cells for [Mg2+]i at 0, 780 and 1600 μM and extracted at 800 s. f Average normalized time course of TRPM7-like current development and inhibition by 500 μM 2-APB as indicated by the black bar ([Mg2+] i  = 0 μM; n = 7)

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Cell Communication and Signaling

ISSN: 1478-811X