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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Deficiency of PI3-Kinase catalytic isoforms p110γ and p110δ in mice enhances the IL-17/G-CSF axis and induces neutrophilia

Fig. 5

Frequencies of cytokine producing T cell subsets are increased in p110γ/δ−/− mice. To determine frequencies of cytokine producing T helper cell (Th cell) subsets, PMA-stimulated splenocytes were measured by flow cytometry. Th cells were gated as singlet live NK1.1 CD3ε+ CD4+ cells and analyzed for intracellular IL-17A, IFN-γ, IL-4, and IL-5 expression. Cytokine secretion by MACs-purified splenic T cells was determined following incubation with indicated concentrations of plate-coated anti-CD3ε or anti-CD3ε/anti-CD28 and IL-17A, IFN-γ, IL-4, and IL-5 concentrations in the supernatants were measured by ELISA. a Cytokine producing Th cell subsets in the spleens of WT, p110γ−/−, p110δ−/−, and p110γ/δ−/− mice. Flow cytometry plots show representative examples of the analysis of IL-17, IFN-γ, IL-4, and IL-5 expression in CD3ε+ CD4+ T cells from one WT and one p110γ/δ−/− mouse and graphs display percentages of cytokine producing Th cell subsets. Bars represent means + SD of pooled data from three independent experiments. b Production of IL-17A, IFN-γ, IL-4, and IL-5 by T cells from WT, p110γ−/−, p110δ−/−, and p110γ/δ−/− mice. Bars represent means + SD of pooled data from three independent experiments carried out in duplicates

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