Fig. 4

Elevated IL-17A levels and increased numbers of IL-17-producing T cells in p110γ/δ^−/− mice. IL-17A serum concentrations were measured using a Bio-Plex assay, and IL-17A-producing T cell subsets in the spleen were analyzed by flow cytometry. a Serum concentration of IL-17A in WT, p110γ^−/−, p110δ^−/−, and p110γ/δ^−/− mice (n = 10). Data were statistically analyzed as described in Fig. 1. b CD3ε⁺CD4⁺ (far-left, top) and CD3ε⁺CD8⁺ T cells (far-left, bottom) were gated from pre-gated singlet live NK1.1⁻ splenic leukocytes and subsequently analyzed for IL-17A expression. Shown are representative examples of the analysis of IL-17A expression in CD3ε⁺CD4⁺ and CD3ε⁺CD8⁺ T cell subsets from WT, p110γ^−/−, p110δ^−/−, and p110γ/δ^−/− mice. c Percentages of IL-17A⁺ subpopulations of CD3ε⁺ CD4⁺ or CD3ε⁺ CD8⁺ T cell subsets in WT, p110γ^−/−, p110δ^−/−, and p110γ/δ^−/− mice (n = 4–10). Data were statistically analyzed as described in Fig. 1