Fig. 2

Increased neutrophil cell death and elevated CD11b⁺ Gr-1^low immature granulocytes in p110γ/δ^−/− mice. (a-c) Cell death of neutrophils was assessed by flow cytometry as measured by an increase in cell permeability of CD3ε⁻CD19⁻ CD11b⁺ Siglec-F⁻ Ly6G⁺ gated neutrophils to 7-AAD. Percentages of 7-AAD⁺ cells of gated neutrophils and total numbers of 7-AAD⁺ neutrophils in (a) BM, (b) blood, and (c) spleen of WT, p110γ^−/−, p110δ^−/−, and p110γ/δ^−/− mice. (d) Differentiating granulocytes in the BM were classified based on their expression of the cell surface markers CD11b and Gr-1. d Percentages (left panel) and total numbers (right panel) of CD11b⁺Gr-1^low and CD11b⁺Gr-1^high cells in WT, p110γ^−/−, p110δ^−/−, and p110γ/δ^−/− mice. Bars represent means + SD of n = 7–8 mice per group. Data were statistically analyzed as described in Fig. 1