Fig. 1

Increased neutrophil numbers in blood, spleen, and lung of p110γ/δ^−/− mice. To determine neutrophil numbers in WT, p110γ^−/−, p110δ^−/−, and p110γ/δ^−/− mice leukocyte suspensions from BM, blood, spleen, and lung were labeled with fluorescent antibodies and analyzed by flow cytometry. a Neutrophils were gated as singlet, live CD3ε⁻ CD19⁻ CD11b⁺ Siglec-F⁻ cells expressing Ly6G. Shown are representative examples of the gating strategy used for the analysis of neutrophils in spleen cell suspensions from one WT (top) and one p110γ/δ^−/− mouse (bottom). Back-gating of gated neutrophils on FSC-A / SSC-A plots (far-right, top and bottom) demonstrate the increased abundance of neutrophils in the p110γ/δ^−/− mouse (far-right bottom) when compared with the WT mouse (far-right, top). (b - e) Percentages and total numbers of gated neutrophils in (b) BM, (c) blood, (d) spleen, and (e) lung of WT, p110γ^−/−, p110δ^−/−, and p110γ/δ^−/− mice. Bars represent means + SD of n = 5–8 (BM, spleen), n = 12 (blood) or n = 4–11 (lung) mice per group. Data were analyzed by One-way ANOVA followed by Bonferroni’s comparison tests for selected pairs of columns. ***P < 0.001, **P < 0.01, *P < 0.05. Asterisks indicate differences in comparison to WT mice