Table 2 Gene names and primer sequences used in real-time PCR experiments
Gene name and symbol | Forward primer | Reverse primer |
|---|---|---|
Progesterone receptor (PgR) | CCCGCCGTCGTAACTTTGG | GTGCCTATCCTGCCTCTCAATC |
Growth regulation in breast cancer 1 (GREB1) | GAGGATGTGGAGTGGAGACC | CAGTACCTCAAAGACCTCGGC |
Trefoil Factor 1(TFFI/pS2) | ACCATGGAGAACAAGGTGA | CCGAGCTCTGGGACTAATCA |
Amphiregulin (AREG) | GTATTTTCACTTTCCGTCTTGTTTTG | CCTGGCTATATTGTCGATTCA |
Forkhead box M1 (FOXM1) | AGCGAGACCCATCAAAGTGG | GGTCTTGGGGTGGGAGATTG |
Estrogen receptor 1 (ESR1/ERα) | TTTATGGGAAAAGGCTCAAA | GACAAAACCGAGTCACATCA |
Estrogen receptor 2 (ESR2/ERβ) | AGAGTCCCTGGTGTGAAGCAAG | GACAGCGCAGAAGTGAGCATC |
FBJ murine osteosarcoma viral oncogene homolog (FOS) | GAATTAACCTGGTGCTGGAT | GAACACACTATTGCCAGGAA |
Peroxisome proliferator-activated receptor gamma (PPARG) | GCAATCAAAGTGGAGCCTGC | CCCTTGCATCCTTCACAAGC |
Hypoxia inducible factor 1, alpha subunit (HIF1A) | CTGCCACCACTGATGAATTA | GTATGTGGGTAGGAGATGGA |
Endothelial PAS domain protein 1 (EPAS1/HIF2α) | GCGCTAGACTCCGAGAACAT | TGGCCACTTACTACCTGACCCTT |
Vascular endothelial growth factor A (VEGFA) | AGGAGGAGGGCAGAATCATCA | CTCGATTGGATGGCAGTAGCT |
DNA-damage-inducible transcript 4 (DDIT4/REDD1) | AGGAAGCTCATTGAGTTGTG | GGTACATGCTACACACACAT |
Nuclear Receptor Subfamily 2 Group F member 1 (NR2F1/COUP-TFI) | TACGTGAGGAGCCAGTACCC | CGATGGGGGTTTTACCTACC |
Chemokine (C-X-C motif) receptor 4 (CXCR4) | GCCTTATCCTGCCTGGTATTGTC | GCGAAGAAAGCCAGGATGAGGA |
Atypical chemokine receptor 3 (ACKR3/CXCR7) | ACAGGCTATGACACGCACTG | ACGAGACTGACCACCCAGAC |
Chemokine (C-X-C motif) ligand 12 (CXCL12) | CTCCTGGGGATGTGTAATGG | GCCTCCATGGCATACATAGG |
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | GGGCATCCTGGGCTACACTG | GGGCATCCTGGGCTACACTG |
TATA box binding protein (TBP) | TGCACAGGAGCCAAGAGTGAA | CACATCACAGCTCCCCACCA |