Table 1 Gene primers list
Target gene | Primer sequence | Â |
|---|---|---|
|  | Forward (5′-3′) | Reverse (5′-3′) |
Gapdh | GGCATTGCTCTCAATGACAA | TGTGAGGGAGATGCTCAGTG |
Alp | GATAACGAGATGCCACCAGAGG | TCCACGTCGGTTCTGTTCTTC |
Runx2 | AGATGATGACACTGCCACCTCTG | GGGATGAAATGCTTGGGAACTGC |
Opn | TGCACCCAGATCCTATAGCC | CTCCATCGTCATCATCATCG |
Osx | CTCTCTGCTTGAGGAAGAAG | GTCCATTGGTGCTTGAGAAG |
Ki67 | CAGACGAGCAAGAGACAAAG | AAGTACGGAGCCTGTATCAC |
SDF-1 | GCTCTGCATCAGTGACGGTA | TAATTTCGGGTCAATGCACA |
Jagged-1 | CTTCAATCTCAAGGCCAGCC | CAGGCGAAACTGAAAGGCAG |
N-cadherin | ACGAGAGGCCTATCCATGCTGAGC | TCAGTCGTCACCACCGCCGTACAT |
Vcam-1 | ATTTTCTGGGGCAGGAAGTT | ACGTCAGAACAACCGAATCC |
Angpt1 | AGGCTTGGTTTCTCGTCAGA | TCTGCACAGTCTCGAAATGG |
Fgfr1IIIb | CTTGACGTCGTGGAACGATCT | AGA ACGGTCAACCATGCAGAG |
Fgfr1IIIc | AGAGACCAGCTGTGATGACC | TGTTACCTGTCTGCGCAGAG |
Fgfr2IIIb | CCCATCCTCCAAGCTGGACTG | CAGAGCCAGCACTTCTGCATTG |
Fgfr2IIIc | CCCATCCTCCAAGCTGGACTG | TCTCACAGGCGCTGGCAGAAC |
Fgfr3IIIb | GGCGCTAACACCACCGAC | TGGCAGCACCACCAGCCAC |
Fgfr3IIIc | TGGAAACTGATGAGGCTGGC | AAACGAGAGACCTTGTGCAC |