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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Fibroblast growth factor 2 supports osteoblastic niche cells during hematopoietic homeostasis recovery after bone marrow suppression

Fig. 3

The proliferation rate and expression of HSC niche-related genes increased by FGF2 in endosteal osteoblasts isolated from 5FU-treated mice. a & b Representative pictures of OB colonies from PBS- or 5FU-treated mice for 4 days, after culture in the presence or absence of FGF2 (50 ng/ml) for 14 days. CFU assay was performed and O.D. values were measured at 540 nm. The O.D value was normalized to the bone weight used for primary cell culture and was presented as a relative O.D. values to the control of ‘without FGF2’ (thus expressed ‘O.D. 540 nm/control’) (c, d, e) The PBS- or 5FU-activated OBs were cultured in the presence of FGF2 at various concentrations (0, 50, 80 ng/ml) for 14 days. Total RNA was isolated, and the expression of HSC niche-related genes Scf, Jagged-1, N-cad was analyzed compared to Gapdh levels by qRT-PCR and presented as ratios to the control (PBS) OBs’ relative value. f, g, h Blocking of FGF receptor signaling by FGFR inhibitor. 5FU-activated OBs were cultured from 5FU-treated mice for 4 days in the absence or presence of SU5402 (5 μM) for 14 days. Total RNA was isolated and the expression of HSC niche-related genes Scf (f), Jagged-1 (g), and N-cad (h) were analyzed against Gapdh levels by qRT-PCR and presented as ratios to the control OBs’ relative value

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