Fig. 6

LASP2 is negatively regulated by LASP1 in colorectal cancer cells and tissues. a Relative mRNA expression of LASP1 and LASP2 in CRC cell lines tested by real time RT-PCR. Error bars represent mean SD averaged from 3 independent experiments. b LASP2 and LASP1 expression level was examined by western blot after transfected with LASP1 vector in SW620 and siRNA in SW480. Immunosignals of protein expression level were quantified by greyscale scanning software and relative protein abundance was normalized by β-tubulin. c Paraffin-embedded CRC samples were stained with anti-LASP1 or anti-LASP2 antibodies in the same position. Graphical illustration was shown in the right of LASP2 expression in the existence of LASP1 in 89 CRC samples