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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Kaiso differentially regulates components of the Notch signaling pathway in intestinal cells

Fig. 5

Kaiso promotes the expression of Jagged-1. a Immunofluorescence staining revealed enhanced Jagged-1 expression and increased numbers of Jagged-1 positive cells (yellow arrowheads), as quantified in the associated graph, in Kaiso Tg crypts. The crypt boundary is indicated with a dotted white line. b Jagged-1 mRNA and (c) protein levels are reduced in stable Kaiso-depleted SW480 cells, as determined by qRT-PCR and western blot, respectively. d Schematic representation of the human JAG1 promoter from −200 bp to +2500 bp of the transcription start site (+1). Arrowheads indicate PCR-amplified regions. Vertical lines represent CpG dinucleotides. CpG Islands (CGI) 1–5 are located −112 to +114, +165 to +540, +547 to +824, +853 to +1458, and +1615 to +1886, respectively. e Kaiso associates with KBS-B, but not KBS-C of the JAG1 promoter. 5’aza-treatement led to loss of Kaiso binding to KBS-B. Asterisk (*) denotes primer dimers. Statistical significance determined by student’s t-test. Error bars are SEM, * p < 0.05

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