Fig. 3
From: Kaiso differentially regulates components of the Notch signaling pathway in intestinal cells
Kaiso inhibits Notch1 expression and N1-ICD levels. a qRT-PCR analysis revealed decreased mNotch1 and mNotch2 in isolated IECs of Kaiso Tg compared to NonTg mice. b IHC revealed reduced N1-ICD protein levels in Kaiso Tg mice. Insets are enlarged images of the area boxed with a dotted line. c Stable Kaiso-depleted (shKaiso) SW480, HCT116, and HT29 cells exhibit increased levels of N1-ICD as determined by western blot (gamma changes employed). d Kaiso overexpression attenuates N1-ICD-mediated transactivation of the 4xCSL artificial promoter in SW480 and HCT116 cells. Cells were co-transfected with 0.5 μg pGLuc-4xCSL, 0.25 μg pCAGGS-N1-ICD, and the indicated amounts of pcDNA3-hKaiso. Statistical significance was determined by student’s t-test or one-way ANOVA with Bonferroni post-test. Error bars are SEM, ns -not significant, *p < 0.05, **p < 0.001, ***p < 0.0001. Scale bar, 50 μm