Fig. 4

Density-dependent shift of proliferation and cell diameter. a Proliferation rate significantly slows at day 3 in cells seeded at 10,000 cells/cm2 (red) but increases in cells seeded at 1,000 cells/cm² (blue; fold increase of 2.1 vs 3.7; *** p-value < 0.001). b Higher density cells have a greater percentage of cells with large diameter (9–10 μm, 19.6% vs 8.2%; *** p-value < 0.001) and fewer small diameter cells (5–6 μm, 26.1% vs 36.2%; *** p-value < 0.001). c Flow cytometric cell scanning contour plot indicating 10,000 cells/cm² seeding density results in a greater proportion of cells exhibiting high forward scatter and FLK1 allophycocyanin conjugate (APC) positivity