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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: Senescence-associated IL-6 and IL-8 cytokines induce a self- and cross-reinforced senescence/inflammatory milieu strengthening tumorigenic capabilities in the MCF-7 breast cancer cell line

Fig. 7

The pro-inflammatory cytokines IL6 and IL8 are required for the maintenance of the migration capacity and stem cell properties in MCF-7 cells. MCF-7 cells were incubated with SCM with or without 1 μg/ml of neutralizing monoclonal antibodies against IL6 (Ref: MAB206, R&D systems) and IL8 (Ref: AF-208-NA, R&D systems). a Wound healing assays showing a decrease in the migration capacity of treated MCF-7 cells with the neutralizing antibodies. The histograms show the quantification of the area of wound healing from at least 10 images taken at 12, 24 and 36 h, using the image J program. Error bars indicate SEM. (***p < 0.001). b Sphere formation assay in the presence of defined medium (EGF and FGF) in MCF-7 cells that were previously treated or not (Control) with SCM alone or in the presence of neutralizing antibodies. The total number of primary, secondary and tertiary spheres per well larger than 100 μm was counted at day 5. Error bars indicate SEM. (***p < 0.001) (n = 2). c Representative micrographs (10×) showing primary, secondary and tertiary generations of mammospheres. Scale bar, 100 μm

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Cell Communication and Signaling

ISSN: 1478-811X