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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Senescence-associated IL-6 and IL-8 cytokines induce a self- and cross-reinforced senescence/inflammatory milieu strengthening tumorigenic capabilities in the MCF-7 breast cancer cell line

Fig. 4

The treatment with IL6 and IL8 induced gene expression and functional changes associated with an EMT program. Gene expression levels of EMT-associated transcription factors Zeb1, Twist 1 and Twist 2 (a) and Snail 1 and Snail2/Slug (b) in MCF-7 cells stimulated with cytokines as indicated and determined by qRT–PCR. The values were normalized to GADPH and relative to control cells (dotted lines). Error bars represent SEM. (*p < 0.05; **p < 0.01; ***p < 0.001) (n = 2). c Adhesion of MCF-7 cells to immobilized-fibronectin after cytokine treatment. Representative micrographs (20×) taken from the plates stained with Crystal violet. The number of adherent cells was counted. Error bars indicate SEM. (*p < 0.05; ** p < 0.01)

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Cell Communication and Signaling

ISSN: 1478-811X