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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Senescence-associated IL-6 and IL-8 cytokines induce a self- and cross-reinforced senescence/inflammatory milieu strengthening tumorigenic capabilities in the MCF-7 breast cancer cell line

Fig. 3

The treatment with IL6 and IL8 increases the migration capacity of MCF-7 cells. a Wound healing assays performed in MCF-7 cells treated with cytokines. Representative micrographs (10×) were taken at 0, 6 and 12 h showing an increased migration capacity of treated MCF-7 cells. Black dotted line indicated the area of the wound. b Quantification of the area of wound healing assay from at least 10 images by using the image J program. Error bars indicate SEM. (**p < 0.01). c Transwell migration assay towards 20% FBS performed in cytokine-treated and control MCF-7 cells. Representative micrographs (20×) were taken from the membrane filter (bottom surface of filters) stained with Crystal violet. d Quantification of transwell migration assay by counting the number of cells present in the lower compartment. Error bars indicate SEM. (*p < 0.05). e Transwell matrigel invasion assay towards 20% FBS performed in cytokine-treated and control MCF-7 cells. The histograms show the number of cells present on the bottom surface of filters from at least 15 images. Error bars indicate SEM. (***p < 0.001) (n = 2)

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Cell Communication and Signaling

ISSN: 1478-811X