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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: The role of apoptosis repressor with a CARD domain (ARC) in the therapeutic resistance of renal cell carcinoma (RCC): the crucial role of ARC in the inhibition of extrinsic and intrinsic apoptotic signalling

Fig. 5

ARC knockdown further enhances topotecan-induced sensitisation towards ABT263-induced apoptosis. a ClearCa-3, −6 and −12 cells were treated with 0.1 μg/ml or 10 μg/ml topotecan for 24 h. Co-treatment with 10 μM ABT263 for the last 3 h of the incubation synergistically enhanced caspase-9 activation as well as mitochondrial depolarisation (JC-1) measured by flow cytometry, compared to single treatment with topotecan or ABT263. b ClearCa-12 cells transduced with non-silencing shRNA or ARC knockdown shRNA were treated with 10 μg/ml topotecan for 24 h, and 10 μM ABT263 was added for the final 3 h of incubation. ARC knockdown further enhanced caspase-9 activation as well as mitochondrial depolarisation following co-treatment compared to cells transduced with non-silencing shRNA. A synergistic effect (SYN) was determined as i1,2 ≥ (i1 + i2) +20%, \where i1,2 = effect of co-treatment, i1 = effect of topotecan and i2 = effect of ABT263

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Cell Communication and Signaling

ISSN: 1478-811X