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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: The role of apoptosis repressor with a CARD domain (ARC) in the therapeutic resistance of renal cell carcinoma (RCC): the crucial role of ARC in the inhibition of extrinsic and intrinsic apoptotic signalling

Fig. 4

Knockdown of ARC sensitises clearCa-12 towards TRAIL-, topotecan- and ABT263-induced apoptosis. ARC knockdown clearCa-12 or control clearCa-12 cells (non-silencing) were treated with (a) TRAIL (100 ng/ml), (b) topotecan (10 μg/ml), or (c) ABT263 (10 μM). ARC knockdown sensitised clearCa-12 cells against the extrinsic apoptotic pathway induced by TRAIL as determined by caspase-8 and caspase-3 activation. Topotecan and ABT263 induced mitochondrial apoptosis as determined by caspase-9 and caspase-3 activation respectively mitochondrial activation. Furthermore, ARC knockdown enhanced caspase-9 activation induced by TRAIL. All values are expressed as the mean ± s.d. *p < 0.05

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Cell Communication and Signaling

ISSN: 1478-811X