TGFBR2-dependent alterations of exosomal cargo and functions in DNA mismatch repair-deficient HCT116 colorectal cancer cells

Table 2 Luminex assay-based analysis of cytokine and chemokine secretion levels in response to exosomal exposure

	Cytokine/Chemokine concentration [pg/ml]
	IL-4	IL-9	IL-10	IL-17	G-CSF	IP-10	PDGF-B	TNF-a	MIF	SCF
pTGFBR2	0.92	6.21	47.03	23.98	18.90	6.64	255.93	13.62	589.72	85.33
dTGFBR2	0.46	3.73	31.42	15.25	10.06	3.83	40.47	8.53	333.29	33.80
Fold change (pTGFBR2/dTGFBR2)	2.00	1.66	1.50	1.57	1.88	1.73	6.32	1.60	1.77	2.52

Fold change values indicate the ratio of secreted cytokine levels by HepG2 recipient cells upon exposure to exosomes derived from pTGFBR2 HCT116 cells in comparison to exosomes isolated from dTGFBR2 donor cells. G-SCF: Granulocyte Colony Stimulating Growth Factor; IP-10: Interferon-Gamma-Inducible Protein 10; PDGF-B: Platelet-derived Growth Factor-B; MIF: Macrophage Migration Inhibitory Factor; SCF: Stem Cell Factor

ISSN: 1478-811X