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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: TGFBR2-dependent alterations of exosomal cargo and functions in DNA mismatch repair-deficient HCT116 colorectal cancer cells

Fig. 1

Characterization of isolated exosomes. a Transmission electron microscopy (TEM) illustrates the size and shape of exosomes (indicated by arrows) isolated from different MSI colorectal cancer cell lines (HCT116, LoVo, KM12, RKO) and from the model cell line HCT116-TGFBR2 (pTGFBR2: TGFBR2-proficient, dTGFBR2: TGFBR2-deficient). Smaller particles represent vesicle fragments resulting from the isolation procedure. Scale bar = 100 nm. b Western blot analysis shows tetraspanin (CD63, CD9) marker expression for whole cell lysates (WCL) of HCT116-TGFBR2 donor cells and lysates of derived exosomes (EXO). ß-actin served as a loading control. c Nanoparticle tracking analysis (NTA) indicates size distribution for vesicles isolated from HCT116-TGFBR2 cells (representative of six independent experiments)

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Cell Communication and Signaling

ISSN: 1478-811X