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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: Integrin-FAK signaling rapidly and potently promotes mitochondrial function through STAT3

Fig. 7

FAK inhibition depresses mitochondrial membrane potential and increases ROS production. a FAK inhibition with FAK14 for 4 h caused a dose-dependent reduction in mitochondrial membrane potential, as shown by confocal images of red TMRM fluorescence in bEnd5 cells. Cells with complete mitochondrial failure are indicated by arrowheads and detectable only by blue nuclear Hoechst staining. b Spectrometry of TMRM in extracts was used to quantify the effects, and shows that 10 μM FAK14 (FAK10) reduced the membrane potential to the same level as FCCP. Values were normalized to nuclear DAPI staining to account for differences in cell numbers. Data are mean ± SEM from 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001. c Bryostatin (BST) dose-dependently preserved mitochondrial membrane potential in the presence of FAK14. N = 3. FAK14 dose-dependently induced formation of d) superoxide (O2 .-) and e) H2O2. N = 3

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