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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Integrin-FAK signaling rapidly and potently promotes mitochondrial function through STAT3

Fig. 1

RGD and αvβ3 integrin blockade suppresses mitochondrial bioenergetics. a Traces from a representative experiment show mitochondrial bioenergetic deficits in mouse brain bEnd5 cells plated on vitronectin (VTN), in the presence of RGD integrin (RGDS) or αvβ3 integrin (P11) blocking peptides, in serum free medium for 4 h, as measured by oxygen consumption rate (OCR) in an XF24 Seahorse Flux Analyzer. Data are means of 5 wells per condition. b Integrin blockade reduced all four measures of cellular respiration relative to control (no VTN) and VTN treated cells. c Cells cultured in XF24 microplates for 4 h on no substrate or on VTN, with or without integrin blockade, seemed similar in number but cells without substrate and in the presence of RGDS were more rounded. The cell number (d) and protein content (e) in such cells, determined without subsequent Seahorse analyses, were not significantly different across all conditions. Cell counts were independently verified. f Cells cultured for 4 h with VTN, laminin (LAM) or fibronectin (FN) as substrate and added to serum-free media showed increased mitochondrial bioenergetics. All data are means ± SEM of 3–4 independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001

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