Attenuation of chemokine receptor function and surface expression as an immunomodulatory strategy employed by human cytomegalovirus is linked to vGPCR US28

Table 3 BRET_max and BRET₅₀ values (mean ± SEM) from BRET donor saturation curves to detect receptor dimerization

Cotransfection	BRET_max (mean ± SEM)	BRET₅₀ (mean ± SEM)	n
CXCR4-Rluc8/CXCR4-mVenus	2.75 ± 0.042	0.11 ± 0.007	3
CXCR4-Rluc8/US28-mVenus	0.54 ± 0.031	0.24 ± 0.036	3
US28-Rluc8/CXCR4-mVenus	0.53 ± 0.023	0.33 ± 0.031	3
CD86-Rluc8/CD86-mVenus	n.d.	n.d.	3
CXCR4-Rluc8/cytopl. mVenus	n.d.	n.d.	3

Curves were fitted using nonlinear regression assuming a one-site hyperbola
Values are derived from pooled netBRET ± SD from three independent experiments, each performed in quadruplicates
Legend: n.d. not to be determined

ISSN: 1478-811X