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Table 2 Radioligand-displacement studies to detect changes in CXCR4 and US28 surface expression

From: Attenuation of chemokine receptor function and surface expression as an immunomodulatory strategy employed by human cytomegalovirus is linked to vGPCR US28

Cotransfection

Used Radioligand

% of max. bound radioligand

(mean ± SEM)

pKi

(mean ± SEM)

n

CXCR4 + mock (control)

[125I]12G5

100 ± 2

8.13 ± 0.07

7

CXCR4 + US28wt

[125I]12G5

74 ± 5 (***)

8.27 ± 0.26 (n.s.)

7

US28wt + mock (control)

[125I]CX3CL1

99 ± 3

8.82 ± 0.08

7

US28wt + CXCR4

[125I]CX3CL1

70 ± 10 (*)

8.71 ± 0.43 (n.s.)

7

  1. Data presented are derived from seven independent experiments, each performed in duplicate wells, presented as mean ± SEM. Statistical analysis was performed using Student’s t test, comparing coexpression with mock-cotransfection (control). *P < 0.05; ***P < 0.001, n.s. not significant
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Cell Communication and Signaling

ISSN: 1478-811X