Fig. 4From: Attenuation of chemokine receptor function and surface expression as an immunomodulatory strategy employed by human cytomegalovirus is linked to vGPCR US28Analysis of heteromerization between CXCR4 and US28 using BRET and BiLC. a BiLC using protomers of Rluc8 (Rluc8N/Rluc8C) to assess receptor dimerization. Columns show the factor of Rluc8 activity measured in mock-transfected cells. Columns represent means ± SEM of at least three independent experiments, each performed in triplicates. b BRET donor saturation curves by cotransfecting a fixed amount of receptor-Rluc8 in presence of increasing amounts of receptor-mVenus constructs. net BRET was calculated by subtracting BRET ratio of donor-only expressing cells. Curves represent pooled net BRET ratios (±SD) from three independent experiments, each performed in quadruplicates. Curves were fitted using least square nonlinear regressions assuming a one site hyperbola. Data from negative controls were additionally fitted using linear regressionBack to article page