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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Attenuation of chemokine receptor function and surface expression as an immunomodulatory strategy employed by human cytomegalovirus is linked to vGPCR US28

Fig. 4

Analysis of heteromerization between CXCR4 and US28 using BRET and BiLC. a BiLC using protomers of Rluc8 (Rluc8N/Rluc8C) to assess receptor dimerization. Columns show the factor of Rluc8 activity measured in mock-transfected cells. Columns represent means ± SEM of at least three independent experiments, each performed in triplicates. b BRET donor saturation curves by cotransfecting a fixed amount of receptor-Rluc8 in presence of increasing amounts of receptor-mVenus constructs. net BRET was calculated by subtracting BRET ratio of donor-only expressing cells. Curves represent pooled net BRET ratios (±SD) from three independent experiments, each performed in quadruplicates. Curves were fitted using least square nonlinear regressions assuming a one site hyperbola. Data from negative controls were additionally fitted using linear regression

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