Fig. 2

EPB41L5 promotes cell invasiveness of a tongue SCC cell line. a Expression of EPB41L5 in the tongue SCC cell lines SCC-9 and SCC-25 was examined by western blotting. b The invasive ability of each tongue SCC cell line was assessed by the Matrigel invasion assay. After 12 h of incubation, invaded cells were fixed and stained with crystal violet. The numbers of cells in six distinct regions of a single chamber were counted. Data are shown as means ± SEM. ***P < 0.001. c SCC-9 cells were transfected with two different sequences of siRNAs against EPB41L5, and then the Matrigel invasion assay was performed. Data are means ± SEM. *P < 0.05. d Cell viabilities of SCC-9 cells with or without the silencing of EPB41L5 was assessed following the exposure to cisplatin and/or radiation. Cisplatin concentrations and radiation doses are as indicated. Data are shown as means ± SEM. ***P < 0.001