Fig. 2

CXCL16 binds to meningioma cells and activates intracellular signaling pathways. a Cy3-labeled s-CXCL16 binds to primary human meningioma cells, while after incubation with Cy3-labeled lactalbumin binding could not be observed (equal exposure times, representative examples). b Stimulation of cultured primary meningioma cells with 10 nM s-CXCL16 (left) yields a time-dependent activation of the signal kinases ERK1/2 and Akt as detected by western blot using antibodies for the phosphorylated kinases (pAkt and pERK). Equal protein loading was confirmed by detection of the non-phosphorylated kinases (Akt and ERK2), respectively. This effect was also observed when a CXCL16-targeting antibody (right, αCXCL16, 1 μg/ml) was used for stimulations. EGF served as positive control, shown are representative examples of at least 3 independent experiments with cultures from different donors