Fig. 5
The N-terminal SH3 domain of Crkl interacts directly with VASP. a schematic diagramm of the Crkl domain organization and the different GST-Crkl and GST-Spec-SH3 fusion proteins used in this study. b Coomassie blue-stained gel of the purified GST-fusion proteins depicted in (a). BSA (4 μg) was loaded onto the same gel to allow relative quantification of the purified proteins. c, d GST pull-down assay with lysates of human platelets (c) or recombinant, purified His6-VASP (d). Equal amounts of platelet lysate (c) or recombinant VASP (d) were incubated with equimolar amounts of the depicted, immobilized GST fusion proteins or GST alone. After extensive washing, precipitated material was analyzed by Western blotting with anti-VASP specific Abs. The position of VASP is indicated on the right hand side by an arrow. The Western blots show the results of one representative experiment (out of five)