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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Vasodilator-Stimulated Phosphoprotein (VASP)-dependent and -independent pathways regulate thrombin-induced activation of Rap1b in platelets

Fig. 2

Translocation of CalDAG-GEFI and Rap1GAP2 is independent of VASP. Platelets from wild-type (WT) or VASP-deficient mice (VASP KO) stimulated or not with thrombin (0.01 U/ml, 30s) were broken by sonication in detergent-free buffer. Lysates were spun at 100,000 g for 1 h. Cytosolic (supernatant) and crude membrane fractions (pellet) were collected and re-suspended in Laemmli buffer. Proteins were separated by 12 % SDS-PAGE, transferred to PVDF membranes, which were subjected to immunoblotting with one of the following Abs: anti-Rap1GAP2, anti-Rap1b or anti-CalDAG-GEFI. The Western blots show the results of one representative experiment (out of three). Protein loading controls (Ponceau S red staining of the blots) are shown in the bottom panels

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