Fig. 5

Gene-expression profiles influenced by TAMs activation. a Heat map showing differential expression of selected genes in Parental (P), Tyro3 (T), Axl (A) and Mertk (M) upon EGF stimulation for 6 and 24 h. RNA-Seq analysis revealed very distinct transcriptomes in EGFR/TAMs cell lines upon activation with mostly upregulated (upper panels) or downregulated (lower panels). b Venn diagrams showing the overlap of genes that significantly changed upon EGF stimulation for 24 h. The numbers represent a list of non-redundant genes with ≥ 5-fold, upregulation (upper panel) or downregulation (lower panel). c, d, e GSEA enrichment plots showing significant enrichment (ES significant at FDR < 25 %) of indicated gene signatures upregulated in Axl (c), Mertk (d), and Tyro3 (d) cells. f Heat map of the enriched genes involved cell invasion, cell adhesion, ECM organization and cell survival that displayed fold change ≥2 in gene expression in Parental (P), Tyro3 (T), Axl (A) and Mertk (M) compared to respective EGF unstimulated controls. The color indicates directionality of change in gene expression (red = increased, white = no change and blue = decreased). g-i RT–qPCR validation of selected genes differentially upregulated upon 24 h EGF stimulation in EGFR/Axl (g), EGFR/Mertk (h), and EGFR/Tyro3 (i). The genes are SPP1 in Axl (g), TMEM40 in Mertk (h), and MYH3 in Tyro3 (i). Expression levels are normalized to β-actin expression levels. Results shown (mean ± SD) are representative data of 3 independent experiments