Fig. 4

Differential phosphokinase-array profiles of activated Tyro3, Axl and Mertk. a Representative images of phospho-kinase array experiments from EGF stimulated parental and EGFR/TAMs total cell lysates. Activation of Tyro3, Axl and Mertk resulted in robust phosphorylation p-ERK1/2 (pT202/pY204, Box1) and p-Akt (pS473, Box2) and whereas only Axl stimulation led to robust phosphorylation of pAkt (pT308, Box3) and pSTAT3 (pS727, Box4). b Semi-quantitative analysis of 2 independent phospho-kinase array experiments showing the fold increase in phosphorylation in EGF stimulated over respective EGF stimulated parental spots. c Comparison of the fold increase in Src family kinases phosphorylation in Tyro3, Axl and Mertk, showing Axl activation results in the highest fold increase across all the Src family kinases phosphorylation (see also in a above, green boxes). d A summary of the signaling pathways downstream of the respective TAM activation