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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Normalization of TAM post-receptor signaling reveals a cell invasive signature for Axl tyrosine kinase

Fig. 3

Phosphoproteomic profiling reveals specific Tyro3, Axl or Mertk dependent changes in protein phosphorylation. Parental and EGFR/TAM CHO cells were stimulated with EGF for the indicated time points. a Representative PathScan® Multiplex immunoblot analysis of the phosphorylation patterns of p90RSK and Akt upon TAMs activation for 0, 5 or 30 min. b Immunoblotting analysis of total protein tyrosine phosphorylation using anti-p-Tyr (pY1000) antibody. c Immunoblotting analysis of Akt substrate phosphorylation using RXX(s/t), RXRXX(s/t) motif antibody. d Immunoblotting analysis of MAPK substrate phosphorylation using (PXsP) motif antibody. e Summary of the KinomeView profiling showing the temporal phosphorylation profiles induced by respective TAM activation. f Immunoblotting analysis of time dependent changes in p-Akt (pT308), p-Akt (pS473) and p-Erk1/2 (pT202/pY204) following 0, 1, 5 10, 15 or 30 min EGF stimulation, with β-actin as the loading control

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Cell Communication and Signaling

ISSN: 1478-811X