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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Erythrocytes do not activate purified and platelet soluble guanylate cyclases even in conditions favourable for NO synthesis

Fig. 1

NO-dependent platelet inhibition is blunted in the presence of RBCs. a ADP-induced platelet aggregation measured in the whole blood, platelet rich plasma (PRP) and washed platelets (WP). Aggregation in whole blood was measured by Multiplate, in PRP and WP by Apact 4004 aggregometer. For whole blood, 300 μl of citrated whole blood and 300 μl saline were added to the test cell together with indicated concentrations of SNP. After three minutes of incubation at 37 °C, aggregation was initiated by ADP (10 μM). Aggregation curves were recorded for 6 min. PRP and WP samples were preincubated for 1 min with indicated concentrations of SNP or iloprost and aggregation was initiated by 10 μm of ADP and monitored for 3 min. Data of platelet aggregation are presented as area under the curve (AUC). b FACS analysis of integrin αIIbβ3 activation (Pac-1 binding) measured in the whole blood, PRP, washed platelets (WP), and WP with washed RBCs (109/ml). Samples were prepared as described under “Materials and Methods”. Data are presented as mean ± SEM, n = 4; + p < 0.05, compared to control, *p < 0.05 compared to Whole blood sample preincubated with DEA-NO

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