Fig. 6

Loss of Gtpbp2 does not affect stability of Dishevelled or Gsk3b. Xenopus embryos were co-injected in the marginal zone of two-cell blastomeres with 1 ng of mRNA encoding myc-Dvl2 or myc-Gsk3b fusion proteins and 250 ng of gfp mRNA (as loading control), along with 40 ng of control or Gtpbp2 morpholino. Embryos were lysed at stage 10.5, run on an 8 % PAGE gel, and western blots were with probed with monoclonal mouse anti-myc 9E10 antisera. Duplicate lanes correspond to independent biological replicates