Fig. 5

Gtpbp2 reduces Axin protein levels in Xenopus embryos. a HA-mcherry and myc-axin mRNAs were co-injected with a morpholino (40 ng) or morpholino + gtpbp2b mRNA (2 ng), into the marginal zone of two cell embryos, and proteins were analyzed by western blot. b Quantitation of myc-Axin levels, shown as mean ± s.e.m of n = 3. c Morpholino (40 ng) or morpholino + gtpbp2 mRNA (2 ng) plus rhodamine-dextran tracer were injected in the marginal zone of two ventral blastomeres of 4 cell stage embryos. The rhodamine-dextran was used to facilitate ventral marginal zone (primarily mesoderm) dissections under a florescent dissection scope at early gastrula stage 10.5, and two of three biological replicate western blots are shown. d Quantitation of endogenous Axin shown as mean ± s.e.m of n = 3