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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Role of PKCtheta in macrophage-mediated immune response to Salmonella typhimurium infection in mice

Fig. 3

PKCtheta deficiency leads to IL-10 hyperproduction of bone marrow-derived macrophages. a, b Bioplex technology was used to analyse secretion responses of the anti-inflammatory cytokines IL-10 (a) and TGFbeta (b) in the supernatant of differentiated and LPS/IFNgamma-polarized macrophages from PKCtheta −/− versus WT mice. Error bars show the mean of at least three independent experiments ± s.e.m. *p < 0.05; ***p < 0.001; c Differentiated BMDM were allowed to rest for 1 h in X-Vivo 20 medium and were then stimulated with LPS/IFNgamma (100 ng/ml and 10 ng/ml, respectively) for 2 and 4 h and whole cell lysates were subjected to immunoblotting against phosphorylated IRF3 and total IRF3. d Analysis of IL-10 serum concentration of WT and PKCtheta −/− mice after infection with S. typhimurium from day 0 to day 4 with Bioplex technology. Error bars show the mean of at least three independent experiments ± s.e.m. **p < 0.01; e IL-10 serum levels from peripheral blood of wild-type featuring PKCtheta −/− mice after challenging mice with intraperitoneal LPS injection as an alternative infection model. Data were collected from at least three experiments and shown as mean ± s.e.m. *p < 0.05; **p < 0.01

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