The arabidopsis cyclic nucleotide interactome

Table 2 Binding properties of the cyclic nucleotide binding protein candidates

Accession	Description	Nucleotide binding	Nucleotide binding domain	Nucleotide binding site	Alignment with CNBD	Putative CNBD	NO PTM	Site of NO PTM
AT3G13920	eukaryotic translation initiation factor 4A1	ATP	58–255	83–90	GAF	174–399	S-nitrosylation
AT3G12780	phosphoglycerate kinase 1	ATP		432–435	CNB GAF	87–286 171–335	S-nitrosylation S-nitrosylation^HR
AT1G42970	glyceraldehyde-3-phosphate dehydrogenase B subunit	NAD (P)	80–243	91–92	CNB GAF	173–323 83–288	S-nitrosylation Y-nitration
AT4G20360	RAB GTPase homolog E1B	GTP	77–278	86–93 148–152 203–206			S-nitrosylation S-nitrosylation^HR Y-nitration
AT3G60750	Transketolase				CNB GAF	213–408 40–232	S-nitrosylation Y-nitration	Y337
AT5G50920	CLPC homologue 1	ATP	294–434 637–818	302–309 645–652
AT4G37930	serine transhydroxymethyltransferase 1				CNB GAF	369–492 262–458	Y-nitration
AT3G14420	Glycolate oxidase 1	FMN	13–355	285–309	GAF	93–276	Y-nitration
AT4G09650	ATP synthase delta-subunit
AT1G09340	chloroplast stem-loop binding protein of 41 kDA	NAD (P)	54–235				S-nitrosylation S-nitrosylation^HR Y-nitration
AT4G27440	protochlorophyllide oxidoreductase B	NAD (P)	84–234 270–366		CNB	77–279	Y-nitration
AT3G01500	carbonic anhydrase 1				GAF	141–345	S-nitrosylation S-nitrosylation^HR Y-nitration	C280

Annotated nucleotide binding domains and nucleotide binding sites of the candidate CNBPs were extracted from the Uniprot and Interpro databases. The alignment of the candidate CNBPs with known CNBDs was extrapolated from Figs. 1 and 2. Evidence of NO-induced PTM and the PTM site was obtained from the literature [79–82]

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