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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Cisplatin-induced mesenchymal stromal cells-mediated mechanism contributing to decreased antitumor effect in breast cancer cells

Fig. 2

a Untreated and 1 μg/ml cisplatin pretreated MSC were stained to detect β-Galactosidase activity at pH 6. We have shown that part of MSC population underwent senescence after pretreatment. Cells were viewed by phase contrast in light microscope. Magnification 42x. b MSC were overnight pretreated with 1 μg/ml cisplatin. Analysis of phosphorylation profiles of kinases and their protein substrates revealed their increased (RSK1/2/3, WNK1, and other) or doubled (p53, c-Jun) concentration. c MSC overnight pretreated with 1 μg/ml cisplatin were analyzed for the relative level of apoptosis-related proteins. The pretreatment with cisplatin caused small changes in expression of both pro-apoptotic and anti-apoptotic proteins. d 48 h pretreatment of MSC with 1 μg/ml cisplatin increased the level of CXCL1, IL-6, IL-8, CCL2 and MIF cytokines released into MSC medium

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