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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Experimental detection of short regulatory motifs in eukaryotic proteins: tips for good practice as well as for bad

Fig. 5

Pipeline for SLiM discovery. Once a candidate sequence location has been identified in a protein, it is evaluated by applying available bioinformatics resources. If the sequence is conserved, accessible to interact and other information is compatible with the motif function, it may pass to experimentation. Both in vitro and in-cell experiments should be undertaken (See Fig. 6 for expanded experimental options). Given a positive outcome of the research it may then be published. On occasion, it may also be of value to publish a negative outcome

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