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Table 2 Overview of discussed methods for identification and characterization of motif based interactions

From: High-throughput methods for identification of protein-protein interactions involving short linear motifs

Method Peptide library size Pros Cons
Combinatorial phage display 1010 Library size No PTMs, various biases
ProP-PD 104–-106 Unbiased No PTMs
Yeast surface display 108 Some PTMs, unbiased Limited library size
Peptide array 102 – 103 PTMs, semi-quantitative Limited coverage
Biased library
Cost of materials
High-density array 105 – 106 Coverage Cost of materials
Protein microarray 10–100 s Semi-quantitative Protein stability
Labor intense set-up
Y2H 106 Low tech No PTMs