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Table 2 Overview of discussed methods for identification and characterization of motif based interactions

From: High-throughput methods for identification of protein-protein interactions involving short linear motifs

Method

Peptide library size

Pros

Cons

Combinatorial phage display

1010

Library size

No PTMs, various biases

ProP-PD

104–-106

Unbiased

No PTMs

Yeast surface display

108

Some PTMs, unbiased

Limited library size

Peptide array

102 – 103

PTMs, semi-quantitative

Limited coverage

Biased library

Cost of materials

High-density array

105 – 106

Coverage

Cost of materials

Protein microarray

10–100 s

Semi-quantitative

Protein stability

Labor intense set-up

Y2H

106

Low tech

No PTMs