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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: L- and D-lactate enhance DNA repair and modulate the resistance of cervical carcinoma cells to anticancer drugs via histone deacetylase inhibition and hydroxycarboxylic acid receptor 1 activation

Fig. 5

Cervical carcinoma cells express HCAR1 and respond to stimulation with lactate. a Immunocytochemical staining of HCAR1; scale bar, 10 μm. b Representative Western blot for the HCAR1 protein level in three cervical carcinoma cell lines (HeLa, Ca Ski and C33A). The HCAR1 protein level was quantified using densitometry and presented as relative means ± SEM of HCAR1/β-actin ratio normalised to HeLa cells, calculated from three independent experiments. c L- and D-lactate inhibit cAMP accumulation in forskolin-stimulated HeLa cells. Cyclic AMP was measured in cells treated with L- or D-lactate for 30 min in the presence of 10 μM forskolin. The results are expressed as the means ± SEM of at least three independent experiments. d Stimulation of Erk phosphorylation by L- or D-lactate is sensitive to PTX and U0126. Serum-starved HeLa cells were pretreated or not with PTX (100 ng/ml) or U0126 (10 μM) and stimulated with L- or D-lactate for 5 min. Representative blots of three independent experiments are shown

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Cell Communication and Signaling

ISSN: 1478-811X