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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: L- and D-lactate enhance DNA repair and modulate the resistance of cervical carcinoma cells to anticancer drugs via histone deacetylase inhibition and hydroxycarboxylic acid receptor 1 activation

Fig. 3

Lactate treatment initiates DNA-PKcs activation. HeLa cells were incubated in the presence or absence of L-lactate or D-lactate for 24 h, exposed to NCS (2 nM) for 30 min, and allowed to recover for 4 h before staining with a phospho-specific antibody directed against Ser2056 of DNA-PKcs. a Immunocytochemical staining of DNA-PKcs phosphorylation at S2056. Each image shows representative microscopic area for the particular treatment from the same experiment. b The graphs show the means ± SEM of the percentage of cells containing more than six foci from three independent experiments. Statistical significance was evaluated using one-way ANOVA followed by Tukey’s test.*P < 0.05, **P < 0.01 and ***P < 0.001 indicate significant differences compared to the untreated cells

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Cell Communication and Signaling

ISSN: 1478-811X